天然产物无细胞生物合成高效平台的构筑及应用研究

31971348
2019
C2102.合成生物学与生物改造技术
李健
面上项目
研究员
上海科技大学
56万元
无细胞蛋白合成;链霉菌;人工合成蛋白系统;无细胞合成生物学;天然产物
2020-01-01到2023-12-31
  • 中英文摘要
  • 结题摘要
  • 结题报告
  • 项目成果
  • 项目参与人
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序号 标题 类型 作者
1 Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology 期刊论文 Ba F;Zhang Y;Ji X;Liu WQ;Ling S;Li J
2 Regulatory part engineering for high-yield protein synthesis in an all-Streptomyces-based cell-free expression system 期刊论文 Xu H;Yang C;Tian X;Chen Y;Liu WQ;Li J
3 一种适于链霉菌无细胞蛋白合成体系的质粒及其构建方法和应用 专利 李健;许慧玲;刘晚秋
4 限制性内切酶的无细胞快速制备研究 期刊论文 刘晚秋;季向阳;许慧玲;卢屹聪;李健
5 Cell-free protein synthesis enables one-pot cascade biotransformation in an aqueous-organic biphasic system 期刊论文 Liu WQ;Wu C;Jewett MC;Li J
6 Stapled NRPS enhances the production of valinomycin in Escherichia coli 期刊论文 Huang S;Ba F;Liu WQ;Li J
7 Recent advances in applying cell-free systems for high-value and complex natural product biosynthesis 期刊论文 Ji X;Liu WQ;Li J
8 Designing modular cell-free systems for tunable biotransformation of L-phenylalanine to aromatic compounds. 期刊论文 Yang C;Liu Y;Liu WQ;Wu C;Li J
9 Combining Cell-Free Expression and Multifactor Optimization for Enhanced Biosynthesis of Cinnamyl Alcohol 期刊论文 Zhang R;Liu WQ;Ling S;Li J
10 Engineering Escherichia coli to Utilize Erythritol as Sole Carbon Source 期刊论文 Ba F;Ji X;Huang S;Zhang Y;Liu WQ;Liu Y;Ling S;Li J
11 Applications of Serine Integrases in Synthetic Biology over the Past Decade 期刊论文 Ba F;Zhang Y;Wang L;Liu WQ;Li J
12 The nonribosomal peptide valinomycin: From discovery to bioactivity and biosynthesis 期刊论文 Huang S;Liu Y;Liu WQ;Neubauer P;Li J
13 Enzyme–Polymer‐Conjugate‐Based Pickering Emulsions for Cell‐Free Expression and Cascade Biotransformation 期刊论文 Lu H;Ouyang J;Liu WQ;Wu C;Li J
14 Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin 期刊论文 Zhuang L;Huang S;Liu WQ;Karim AS;Jewett MC;Li J
15 Engineered microbes: Making valuable chemicals from waste gases 期刊论文 Li J
16 SYMBIOSIS: Synthetic manipulable biobricks via orthogonal serine integrase systems 期刊论文 Ba F;Liu Y;Liu WQ;Tian X;Li J
17 Plug-and-play functionalization of protein–polymer conjugates for tunable catalysis enabled by genetically encoded “click” chemistry 期刊论文 Liu Y;Ba F;Liu WQ;Wu C;Li J
18 Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan 期刊论文 Tian X;Liu WQ;Xu H;Ji X;Liu Y;Li J
19 Translation related factors improve the productivity of a Streptomyces-based cell-free protein synthesis system 期刊论文 Xu H;Liu WQ;Li J
20 Cell-Free Biosynthesis of Lysine-Derived Unnatural Amino Acids with Chloro, Alkene, and Alkyne Groups 期刊论文 Chen Y;Liu WQ;Zheng X;Liu Y;Ling S;Li J
21 Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold 期刊论文 Zhang Y;Liu WQ;Li J
22 Establishing a eukaryotic Pichia pastoris cell-free protein synthesis system 期刊论文 Zhang L;Liu WQ;Li J
23 Cell-free metabolic engineering enables selective biotransformation of fatty acids to value-added chemicals 期刊论文 Liu Y;Liu WQ;Huang S;Xu H;Lu H;Wu C;Li J
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