基于光镊辅助荧光成像和发光限域型808nm激发上转换FRET纳米探针标记的单细胞分析方法研究

21904102
2019
B0404.化学与生物传感
李诚予
青年科学基金项目
副教授
武汉科技大学
25万元
单细胞分析;荧光标记;光镊;发光限域型上转换纳米颗粒;FRET
2020-01-01到2022-12-31
  • 中英文摘要
  • 结题摘要
  • 结题报告
  • 项目成果
  • 项目参与人
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序号 标题 类型 作者
1 Incorporating luminescence-concentrating upconversion nanoparticles and DNA walkers into optical tweezers assisted imaging: a highly stable and ultrasensitive bead supported assay 期刊论文 Cheng-Yu Li;Ting Zhang;Ya-Feng Kang;Chu-Bo Qi;Bei Zheng;Chun-Miao Xu;Yi Lin;Dai-Wen Pang;Hong-Wu Tang
2 Biomimetic Chip Enhanced Time-Gated Luminescent CRISPR-Cas12a Biosensors under Functional DNA Regulation 期刊论文 Cheng-Yu Li;Bei Zheng;Li-Li Lu;Wen-Kai Fang;Ming-Qiu Zheng;Jia-Ling Gao;Liu Yuheng;Dai-Wen Pang;Hong-Wu Tang
3 A Self-Made Optical Tweezers Integrated Upconversion Luminescence Confocal Scanning Instrument Enables Stable and Noninvasive Long-Term In Situ Imaging a Single Suspension Cell Under Exceptionally Efficient Luminescent Resonance Energy Transfer Sensing 期刊论文 Cheng-Yu Li;Jun-Xian Liu;Meng-Kun Xin;Jing-Wei He;Ya-Ling Chen
4 Photo-gated and self-powered three-dimensional DNA motors with boosted biostability for exceptionally precise and efficient tracing of intracellular survivin mRNA 期刊论文 Yu-Heng Liu;Jia-Ling Gao;Jun-Xian Liu;Da Liu;Wen-Kai Fang;Bei Zheng;Hong-Wu Tang;Cheng-Yu Li
5 Upconversion Luminescence-Initiated and GSH-Responsive Self-Driven DNA Motor for Automatic Operation in Living Cells and In Vivo. 期刊论文 Cheng-Yu Li;Jun-Xian Liu;Liu Yuheng;Jia-Ling Gao;Ya-Ling Chen;Jing-Wei He;Meng-Kun Xin;Da Liu;Bei Zheng;Xiao-Ming Sun
6 Monitoring of viral myocarditis injury using an energy-confined upconversion nanoparticle and nature-inspired biochip combined CRISPR/Cas12a-powered biosensor 期刊论文 Li-Li Lu;Chao-Zhi Li;Heng-Zhong Guo;Da Liu;Hong-Wu Tang;Bei Zheng;Cheng-Yu Li
7 一种单细胞分析系统及方法和应用 专利 李诚予
8 A dual DNA tetrahedrons and MnO2 nanosheets sustained entropy-driven DNA amplifier enables high-performance operation in live cells and bodies under a light-gated manner 期刊论文 Yu-Heng Liu;Jun-Xian Liu;Jia-Ling Gao;Hong-Wu Tang;Cheng-Yu Li
9 Smart NIR light-gated CRISPR/Cas12a fluorescent biosensor with boosted biological delivery and trans-cleavage activity for high-performance in vivo operation. 期刊论文 Jun-Xian Liu;Xiao-Ming Sun;Da Liu;Yu-Heng Liu;Cheng-Yu Li
10 Holographic Optical Tweezers and Boosting Upconversion Luminescent Resonance Energy Transfer Combined Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12a Biosensors 期刊论文 Cheng-Yu Li;Bei Zheng;Jiang-Tao Li;Jia-ling Gao;Yu-Heng Liu;Dai-Wen Pang;Hong-Wu Tang
11 Light-gated cascade amplification DNA circuit under the encapsulation of a natural protein-derived facilitate nanocarrier for spatiotemporal sensing in living cells 期刊论文 Jun-Xian Liu;Xiao-Ming Sun;Meng-Kun Xin;Ya-Ling Chen;Jing-Wei He;Da Liu;Cheng-Yu Li
12 An exceptional and universal DNA walker amplified “one-to-many” CRISPR/Cas12a-mediated fluorescent biosensor for ultrasensitive detection of non-DNA biomarkers 期刊论文 Cheng-Yu Li;Jun-Xian Liu;Yu-Heng Liu;Jia-Ling Gao;Bei Zheng;Da Liu
13 Integrating 808 nm Light-Excited Upconversion Luminescence Powering with DNA Tetrahedron Protection: An Exceptionally Precise and Stable Nanomachine for Intracelluar MicroRNA Tracing 期刊论文 Cheng-Yu Li;Bei Zheng;Ya-Feng Kang;Hong-Wu Tang;Dai-Wen Pang
14 A boosting upconversion luminescent resonance energy transfer and biomimetic periodic chip integrated CRISPR/Cas12a biosensor for functional DNA regulated transduction of non-nucleic acid targets 期刊论文 Cheng-Yu Li;Bei Zheng;Yu-Heng Liu;Jia-Ling Gao;Ming-Qiu Zheng;Dai-Wen Pang;Hong-Wu Tang
15 Light-Activated and Self-Driven Autonomous DNA Nanomachine Enabling Fluorescence Imaging of MicroRNA in Living Cells with Exceptional Precision and Efficiency 期刊论文 Jia-Ling Gao;Yu-Heng Liu;Bei Zheng;Jun-Xian Liu;Wen-Kai Fang;Da Liu;Xiao-Ming Sun;Hong-Wu Tang;Cheng-Yu Li
16 A Photoresponsive and Metal–Organic Framework Encapsulated DNA Tetrahedral Entropy-Driven Amplifier for High-Performance Imaging Intracellular MicroRNA 期刊论文 Jia-Ling Gao;Liu Yuheng;Jun-Xian Liu;Hong-Wu Tang;Cheng-Yu Li
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