编程可控的基因增变器及其在酿酒酵母基因组连续进化中的应用

21808199
2018
B0812.生物化工与合成生物工程
连佳长
青年科学基金项目
研究员
浙江大学
25万元
代谢工程;基因组连续进化;多基因调控技术;合成生物学;基因增变器
2019-01-01到2021-12-31
  • 中英文摘要
  • 结题摘要
  • 结题报告
  • 项目成果
  • 项目参与人
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序号 标题 类型 作者
1 Construction of a series of episomal plasmids and their application in the development of an efficient CRISPR/Cas9 system in Pichia pastoris 期刊论文 Gu Yang;Gao Jucan;Cao Mingfeng;Dong Chang;Lian Jiazhang;Huang Lei;Cai Jin;Xu Zhinan
2 Cloning and characterization of a panel of mitochondrial targeting sequences for compartmentalization engineering in Saccharomyces cerevisiae 期刊论文 Dong Chang;Shi Zhuwei;Huang Lei;Zhao Huimin;Xu Zhinan;Lian Jiazhang
3 Efficient production of S-adenosyl-l-methionine from dl-methionine in metabolic engineered Saccharomyces cerevisiae 期刊论文 Liu Wei;Tang Dandan;Shi Rui;Lian Jiazhang;Huang Lei;Cai Jin;Xu Zhinan
4 Construction of a Stable and Temperature-Responsive Yeast Cell Factory for Crocetin Biosynthesis Using CRISPR-Cas9. 期刊论文 Liu Tengfei;Dong Chang;Qi Mingming;Zhang Bei;Huang Lei;Xu Zhinan;Lian Jiazhang
5 一种增加基因组突变率的碱基编辑系统及碱基编辑方法 专利 连佳长;潘颖佳;夏思杨;董昌
6 一种高表达细胞色素P450的重组酵母及其应用 专利 连佳长;江丽红;董昌;刘腾飞
7 Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae 期刊论文 Liu Tengfei;Huang Ying;Jiang Lihong;Dong Chang;Gou Yuanwei;Lian Jiazhang
8 PCR & Go: A Pre-installed Expression Chassis for Facile Integration of Multi-Gene Biosynthetic Pathways 期刊论文 Qi Mingming;Zhang Bei;Jiang Lihong;Xu Saijuan;Dong Chang;Du Yi-Ling;Zhou Zhan;Huang Lei;Xu Zhinan;Lian Jiazhang
9 A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae 期刊论文 Dong Chang;Jiang Lihong;Xu Saijuan;Huang Lei;Cai Jin;Lian Jiazhang;Xu Zhinan
10 Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping 期刊论文 Jiazhang Lian;Carl Schultz;Mingfen Cao;Mohammad HamediRad;Huimin Zhao
11 Identification of novel metabolic engineering targets for S-adenosyl-L-methionine production in Saccharomyces cerevisiae via genome-scale engineering 期刊论文 Dong Chang;Schultz J. Carl;Liu Wei;Lian Jiazhang;Huang Lei;Xu Zhinan;Zhao Huimin
12 Efficient production of lycopene from CO2 via microbial electrosynthesis 期刊论文 Haoliang Wu;Haojie Pan;Zhongjian Li;Tengfei Liu;Folin Liu;Siyuan Xiu;Jia Wang;Hanqing Wang;Yang Hou;Bin Yang;Lecheng Lei;Jiazhang Lian
13 Combined genome editing and transcriptional repression for metabolic pathway engineering in Corynebacterium glutamicum using a catalytically active Cas12a 期刊论文 Liu Wei;Tang Dandan;Wang Haijiao;Lian Jiazhang;Huang Lei;Xu Zhinan
14 Functional expression of eukaryotic cytochrome P450s in yeast 期刊论文 Jiang Lihong;Huang Lei;Cai Jin;Xu Zhinan;Lian Jiazhang
15 酿酒酵母代谢工程技术 期刊论文 江丽红;董昌;黄磊;徐志南;连佳长
16 Random Base Editing for Genome Evolution in Saccharomyces cerevisiae 期刊论文 Yingjia Pan;Siyang Xia;Chang Dong;Haojie Pan;Jin Cai;Lei Huang;Zhinan Xu;Jiazhang Lian
17 Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization 期刊论文 Haojie Pan;Jia Wang;Haoliang Wu;Zhongjian Li;Jiazhang Lian
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