P450脂肪酸脱羧酶的定向进化及催化机理研究
31770844
2017
C0505.蛋白质、多肽与酶生物化学
徐卉芳
面上项目
副研究员
中国科学院青岛生物能源与过程研究所
60万元
结构—功能关系;酶活性;酶催化机制;P450脂肪酸脱羧酶;定向进化
2018-01-01到2021-12-31
- 中英文摘要
- 结题摘要
- 结题报告
- 项目成果
- 项目参与人
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| 序号 | 标题 | 类型 | 作者 |
|---|---|---|---|
| 1 | Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening towards Improved Catalytic Activity | 期刊论文 | Xu Huifang;Liang Weinan;Ning Linlin;Jiang Yuanyuan;Yang Wenxia;Wang Cong;Qi Feifei;Ma Li;Du Lei;Fourage Laurent;Zhou Yongjin J.;Li Shengying |
| 2 | Method for Detecting Hydrogen Peroxide Conversion Activity of An Enzyme | 专利 | Fourage Laurent;Li Shengying;Xu Huifang;Ning Linlin |
| 3 | Production of a-Olefins | 专利 | Fourage Laurent;Laeuffer Frederic;Strub Henri;Wang Yun;Xu Jian;Xu Huifang;Li Shengying |
| 4 | Improved Cytochrome P450 Fatty Acid Decarboxylases | 专利 | Li Shengying;Xu Huifang;Ning Linlin;Fourage Laurent |
| 5 | Biochemical characterization of three new alpha-olefin-producing P450 fatty acid decarboxylases with a halophilic property | 期刊论文 | Jiang Yuanyuan;Li Zhong;Wang Cong;Zhou Yongjin J.;Xu Huifang;Li Shengying |
| 6 | Establishing an enzyme cascade for one-pot production of alpha-olefins from low-cost triglycerides and oils without exogenous H2O2 addition | 期刊论文 | Jiang Yuanyuan;Li Zhong;Zheng Shanmin;Xu Huifang;Zhou Yongjin J.;Gao Zhengquan;Meng Chunxiao;Li Shengying |
| 7 | In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases | 期刊论文 | Xu Huifang;Ning Linlin;Yang Wenxia;Fang Bo;Wang Cong;Wang Yun;Xu Jian;Collin Severine;Laeuffer Frederic;Fourage Laurent;Li Shengying |
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